S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Arr1p	YPR200C	PubMed Wysocki R et al., Mol Biol Cell, 2004 May;15(5):2049-60	Direct	ChIP	N/A	S288c	N/A
		PubMed Menezes RA et al., FEBS Lett, 2004;():Menezes RA et al., FEBS Lett, 2004;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	arsenic stress (arsenate and arsenite)
		PubMed Haugen AC et al., Genome Biol, 2004;5(12):R95	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	Synthetic complete medium after 2h exposure to 100 microM AsIII (30.C).
		PubMed Ilina Y et al., Biochem J, 2008 Nov 1;415(3):467-75	Direct	EMSA	N/A	S288c	in vitro
		PubMed Amaral C et al., PLoS One, 2013 Dec 16;8(12):e83328	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	BY4741 yap8 with plasmid containing Yap8bZIPL26A protein were collected at early log-phase and exposed to 1mM arsenate (Na2HAsO4.7H2O) for 1h.
		PubMed Kumar NV et al., Mol Cell Biol, 2015 Dec 28;36(6):913-22	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	DELTAyap8 cells expressing GFP-Yap8 or GFPâ€“Yap8-C132A/C274A were grown overnight at 30.C in YNB medium with appropriate selection. The overnight cultures were inoculated to fresh medium at an optical density at 600 nm (OD600) of 0.4 and grown until the exponential phase at 30.C. After taking a null sample and exposing the cells to 0.5 mM As(III), samples were collected at 30, 60, 120 min.
		PubMed Menezes RA et al., Biochim Biophys Acta Gene Regul Mech, 2017 Apr;1860(4):472-481	Direct	ChIP	N/A	BY4742	YPD + 1Â mM As(V) for 1Â h
		PubMed da Silva SM et al., Biochim Biophys Acta Gen Subj, 2018 Oct;1862(10):2152-2161	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	YPD + 2â€¯mM arsenic