The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Arr1p
|
YPR201W
|
PubMed
Wysocki R et al., Mol Biol Cell, 2004 May;15(5):2049-60Wysocki R et al., Mol Biol Cell, 2004 May;15(5):2049-60 |
Direct |
ChIP |
N/A |
S288c |
Exponential-phase cells cultivated in YNB growth medium |
PubMed
Menezes RA et al., FEBS Lett, 2004;():Menezes RA et al., FEBS Lett, 2004;(): |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
arsenic stress (arsenate and arsenite) |
PubMed
Di Y et al., J Cell Sci, 2007;():Di Y et al., J Cell Sci, 2007;(): |
Indirect |
lacZ - wt vs TF overexpression |
Positive |
S288c |
As(III) exposure |
PubMed
Mok YG et al., FEBS Lett, 2008 Mar 19;582(6):916-24Mok YG et al., FEBS Lett, 2008 Mar 19;582(6):916-24 |
Direct |
One-hybrid assay |
N/A |
S288c |
in vitro |
PubMed
Ilina Y et al., Biochem J, 2008 Nov 1;415(3):467-75Ilina Y et al., Biochem J, 2008 Nov 1;415(3):467-75 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Ferreira RT et al., Biol Open, 2015 Aug 14;4(9):1122-31Ferreira RT et al., Biol Open, 2015 Aug 14;4(9):1122-31 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Cells were grown to exponential phase cultures (OD600 0.5±0.05) and exposed to 1.5 mM As(III) for 90 min |
PubMed
Kumar NV et al., Mol Cell Biol, 2015 Dec 28;36(6):913-22Kumar NV et al., Mol Cell Biol, 2015 Dec 28;36(6):913-22 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
DELTAyap8 cells expressing GFP-Yap8 or GFP–Yap8-C132A/C274A were grown overnight at 30.C in YNB medium with appropriate selection. The overnight cultures were inoculated to fresh medium at an optical density at 600 nm (OD600) of 0.4 and grown until the exponential phase at 30.C. After taking a null sample and exposing the cells to 0.5 mM As(III), samples were collected at 30, 60, 120 min. |
PubMed
da Silva SM et al., Biochim Biophys Acta Gen Subj, 2018 Oct;1862(10):2152-2161da Silva SM et al., Biochim Biophys Acta Gen Subj, 2018 Oct;1862(10):2152-2161 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
BY4741 |
YPD + 2 mM arsenic |
|