PubMed
Rottensteiner H et al., J Biol Chem, 2003 Jul 25;278(30):27605-11Rottensteiner H et al., J Biol Chem, 2003 Jul 25;278(30):27605-11
Indirect
lacZ - wt vs TF mutant
Positive
S288c
Late exponential phase cells from overnight pre-cultures consisting of YP medium and 5% (w/v) D-glucose were transferred to 100-ml conical flasks with 50 ml of YP medium containing both 0.2% (w/v) oleic acid (Merck) and 0.02% (w/v) Tween 80 (Sigma-Aldrich) that were adjusted to pH 7.0 with NaOH, 0.05% D-glucose, and 75 ?g/ml ampicillin to an absorbance of A600 = 0.2. Cultures were grown at 30 °C
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
