PubMed
Pierce M et al., Mol Cell Biol, 1998 Oct;18(10):5970-80Pierce M et al., Mol Cell Biol, 1998 Oct;18(10):5970-80
Direct
Northern blot - wt vs mutant promoter sequence
Positive
S288c
sporulated cultures at 10 h postinduction (Logarithmically growing cultures were used to inoculate YEPA, the cultures were expanded overnight at 30°C to a density of 1 3 107 cells/ml (4 to 5 generations), and cells were collected by centrifugation, washed once in 2% potassium acetate, and resuspended at 4 3 107 cells/ml in SM (2% potassium acetate, 10 mg of adenine/ml, 5 mg of histidine/ml, 30 mg
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314