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Regulation Reference(s)
The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor |
Target
ORF/Genes |
References |
Evidence
Code |
Evidence
Experiment |
Association
Type |
Strain |
Environmental
Condition |
|
Aft1p
|
YKL220C
|
PubMed 
Yamaguchi-Iwai Y et al., EMBO J, 1995 Mar 15;14(6):1231-9Yamaguchi-Iwai Y et al., EMBO J, 1995 Mar 15;14(6):1231-9 |
Indirect |
Northern blot - wt vs TF overexpression |
Positive |
S288c |
cells grown with iron deprivation, expressing a constitutive AFT1 allele |
|
PubMed
Yamaguchi-Iwai Y et al., EMBO J, 1996 Jul 1;15(13):3377-84Yamaguchi-Iwai Y et al., EMBO J, 1996 Jul 1;15(13):3377-84 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
|
PubMed
Martins LJ et al., J Biol Chem, 1998 Sep 11;273(37):23716-21Martins LJ et al., J Biol Chem, 1998 Sep 11;273(37):23716-21 |
Direct |
DNA footprinting |
Positive |
S288c |
Minimal medium; mid-log phase |
|
PubMed
Courel M et al., Mol Cell Biol, 2005 Aug;25(15):6760-71Courel M et al., Mol Cell Biol, 2005 Aug;25(15):6760-71 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
SCMC01 |
Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C. |
|
PubMed
Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
YPD medium + 200mM hydroxyurea |
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