S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Aft1p	YMR058W	PubMed Yamaguchi-Iwai Y et al., EMBO J, 1995 Mar 15;14(6):1231-9	Indirect	lacZ - wt vs TF overexpression	Positive	S288c	cells grown with iron deprivation, expressing a constitutive AFT1 allele
		PubMed Yamaguchi-Iwai Y et al., EMBO J, 1996 Jul 1;15(13):3377-84	Direct	In vivo footprinting WT vs TF mutant vs TF overexpressing mutant	N/A	S288c	iron-starved medium
			Indirect	lacZ - wt vs TF overexpression	Positive	S288c	iron-starved medium
		PubMed Muchardt C et al., EMBO J, 1996 Jul 1;15(13):3394-402	Direct	In vivo footprinting WT vs TF mutant vs TF overexpressing mutant	N/A	S288c	iron-repleted medium (100mM Ferric ammonium sulfate)
		PubMed Lin SJ et al., J Biol Chem, 1997;():Lin SJ et al., J Biol Chem, 1997;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Gross C et al., J Biol Chem, 2000 Oct 13;275(41):32310-6	Direct	DNA footprinting	Positive	S288c	low copper complete medium
		PubMed Blaiseau PL et al., J Biol Chem, 2001 Sep 7;276(36):34221-6	Indirect	Northern blot - wt vs TF mutant	Positive	CM3260	Iron depletion; cells in log phase grown in iron and copper limiting medium without iron addition
			Indirect	Northern blot - wt vs TF mutant	Positive	CM3260	Iron repletion; cells in log phase grown in iron and copper limiting medium supplemented with 100 microM iron
			Indirect	lacZ - wt vs TF mutant	Positive	CM3260	Iron depletion; cells in log phase grown in iron and copper limiting medium without iron addition
			Indirect	lacZ - wt vs TF mutant	Positive	CM3260	Iron repletion; cells in log phase grown in iron and copper limiting medium supplemented with 100 microM iron
		PubMed Rutherford JC et al., Proc Natl Acad Sci U S A, 2001 Dec 4;98(25):14322-7	Direct	EMSA	N/A	S288c	in vitro
		PubMed De Freitas JM et al., J Biol Chem, 2004 Feb 6;279(6):4450-8	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	YPD medium; Cell were grown to early log phase;
		PubMed Courel M et al., Mol Cell Biol, 2005 Aug;25(15):6760-71	Indirect	Microarray WT vs TF mutant	Positive	Y14438	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
			Indirect	Northern blot WT vs TF mutant	Positive	Y14438	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
			Indirect	Northern blot WT vs TF mutant	Positive	Y14438	Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C.
			Indirect	Northern blot - wt vs TF mutant	Positive	SCMC01	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
			Indirect	Northern blot - wt vs TF mutant	Positive	SCMC01	Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C.
			Indirect	Microarray analysis - wt vs TF mutant	Positive	SCMC01	Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
		PubMed Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Pagani MA et al., Mol Microbiol, 2007 Jul;65(2):521-37	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	aft1DELTA versus wt: Cells exposed to 5mM ZnCl2 for 30 minutes in YPD media
		PubMed Hausmann A et al., J Biol Chem, 2008 Mar 28;283(13):8318-30	Indirect	promoter assay	Positive	BY4741	wt versus TF overexpression SD media mid exp cells Fe-repleted cells
		PubMed KumÃ¡novics A et al., J Biol Chem, 2008 Apr 18;283(16):10276-86	Direct	ChIP	N/A	S288c	low iron medium (CM + 40 ?m BPS)
		PubMed Ihrig J et al., Eukaryot Cell, 2010 Mar;9(3):460-71	Indirect	GFP - wt vs TF overexpression	Positive	S288c	50mM bathophenanthroline (Fe depletion)
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Berthelet S et al., Genetics, 2010 Jul;185(3):1111-28	Direct	ChIP	N/A	S288c	YPD
		PubMed Miao R et al., Biochemistry, 2011 Apr 5;50(13):2660-71	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Iron depletion
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	iron repletion, constitutive TF
		PubMed PÃ©rez-Sampietro M et al., Fungal Genet Biol, 2014;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	WT and DELTAaft1 cells growing exponentially in YPD liquid medium and then treated with 2 mM selenite for 3h or 6h. SNR19 was employed as loading control.
		PubMed Kingsbury JM et al., Hum Mol Genet, 2016 Aug 15;25(16):3524-3538	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	IDP1R148H (analogous to human Idh1R132) with strong GAL1 promoter in S288c cells grown in Synthetic Complete Galactose + URA 24h of incubation, with high copy of AFT1
		PubMed Gaspar-Cordeiro A et al., FEBS J, 2018 May;285(10):1861-1872	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4742	SC medium
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4742	SC medium + 2mM CuSO4
			Indirect	Western blot - wt vs mutant promoter sequence	Positive	BY4742	SC medium
			Indirect	Western blot - wt vs mutant promoter sequence	Positive	BY4742	SC medium + 2mM CuSO4
		PubMed Caballero-Molada M et al., Biochem J, 2018 Apr 30;475(8):1523-1534	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	SD medium, mid exponential phase
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Cells cultured to mid-exponential phase in SD medium with amino acid supplementation
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Cells cultured to mid-exponential phase in SD medium
		PubMed Pujol-Carrion N et al., Biochim Biophys Acta Mol Cell Res, 2021 Apr;1868(5):118974	Indirect	lacZ - wt vs TF mutant	Positive	CML128	SD medium
			Indirect	lacZ - wt vs TF mutant	Positive	CML128	SD medium Fe depleted
			Indirect	lacZ - wt vs TF mutant	Positive	CML128	SD medium Fe repleted (10mM, 6h)
		PubMed Jordá T et al., J Fungi (Basel), 2021 Oct 25;7(11):	Indirect	RT-PCR - wt vs TF overexpression	Positive	BY4741	SC medium
		PubMed Jordá T et al., J Fungi (Basel), 2021 Oct 25;7(11):	Indirect	RT-PCR - wt vs TF overexpression	Positive	BY4741	SC medium with 100 microM BPS for 9 h (Iron Deficiency)