S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Aro80p	YHR137W	PubMed Iraqui I et al., Mol Cell Biol, 1999 May;19(5):3360-71	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	urea and tryptophan
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	SC medium; mid-log phase
		PubMed Chen H et al., Genes Dev, 2006 May 1;20(9):1150-61	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	low cell density and aromatic alcohol (tryptophol); cells inoculated in low ammonium dextrose medium for 4 h
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Godard P et al., Mol Cell Biol, 2007 Apr;27(8):3065-86	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Urea as nitrogen source + 1h 5mM tyrosine
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	wt and DELTAaro80 strains containing a pARO9-lacZ reporter plasmid were cultured in synthetic complete medium with amino acids and treated with 3-AT or rapamycin or not treated
		PubMed Lee K et al., Mol Microbiol, 2013 Jun;88(6):1120-34	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	BY4741 aro80DELTA cells expressing pRS415ADH-Aro80-HA were grown in minimal medium until early log phase and then the medium was changed to YPD. The cells were treated or not with 0.2 microg mlâˆ’1 rapamycin for 1 h
		PubMed Kim B et al., Biotechnol Bioeng, 2014 Jan;111(1):115-24	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	SC-Leu_Trp_Ura medium
		PubMed Lee K et al., Biochem Biophys Res Commun, 2013 Aug 16;438(1):43-7	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YPD medium 39.C
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C