PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Positive
BY4741
YNB media supplemented with 20 mg/L methionine
PubMed
Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014
Direct
EMSA
N/A
in vitro
EMSAs with large DNA fragments. The 32P-labeled DNA fragments were incubated with 0, 50 and 200 ng of a recombinant derivative of Cbf1 containing the bHLH domain fused to a polyhistidine tag (hisCbf1 BD).
Direct
ChIP
N/A
W303
Isogenic strain expressing Myc-tagged Cbf1 was grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+. Binding to PDC6 upstream region (from -455 to -306 relative to ATG)
Direct
ChIP
N/A
W303
Isogenic strain expressing Myc-tagged version of Cbf1 was grown to early log phase in YPD medium and exposed to 0.5mM Cd2+.
Indirect
RT-PCR - wt vs TF mutant
Positive
W303
wt vs DELTAcbf1: cells were grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000
Direct
ChIP-exo
N/A
CEN.PK 113-5D
Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
Direct
ChIP-exo
N/A
CEN.PK 113-5D
Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
Direct
ChIP-exo
N/A
CEN.PK 113-5D
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h