PubMed
Laskar S et al., Mol Cell Biol, 2015 Jan;35(2):437-50Laskar S et al., Mol Cell Biol, 2015 Jan;35(2):437-50
Indirect
RT-PCR - wt vs TF mutant
Positive
S288c
wt and cells with overexpression of Myc-tagged Cup9. Cup9 was induced by adding galactose to a final concentration of 2%. The overnight culture grown in galactose medium was used as a secondary inoculum in galactose-containing medium and was grown for an additional 5 h (until the mid-log phase) before the isolation of RNA.