S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Ecm22p	YLR056W	PubMed Vik A et al., Mol Cell Biol, 2001 Oct;21(19):6395-405	Indirect	lacZ - wt vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with 40 microg/mL lovastatin
			Indirect	lacZ - wt vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with or without 40 microg/mL lovastatin
		PubMed Davies BS et al., Mol Cell Biol, 2005 Aug;25(16):7375-85	Indirect	lacZ - wt vs TF mutant	Positive	JRY7180	Complete synthetic medium (CSM; 0.67%Difco yeast nitrogen base without amino acids, complete supplemental mixture minus appropriate amino acids) containing 2% glucose; mid-log phase.
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Davies BS et al., Genetics, 2006 Sep;174(1):191-201	Indirect	RT-PCR - wt vs TF mutant	Positive	W303	Cells were grown aerobically in complete synthetic medium containing 2% glucose with ketoconazole
			Indirect	RT-PCR - wt vs TF mutant	Negative	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions)
			Indirect	RT-PCR - wt vs TF mutant	Positive	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions) with hemin
			Indirect	RT-qPCR (WT vs TF mutant)	Positive	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions)
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Gallo-Ebert C et al., J Biol Chem, 2013 Dec 6;288(49):35466-77	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Cells were grown to exponential phase at 30 .C in synthetic medium. Cells with pRS-ERG3 plasmid that contained ( ) or lacked ( ) all three SRE/AR1 c elements.
		PubMed Woods K et al., Mol Microbiol, 2016 Feb;99(3):512-27	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Medium with 2% galactose
		PubMed Wang SQ et al., Lett Appl Microbiol, 2018 Nov;67(5):484-490	Indirect	RNA-seq - wt vs TF mutant	Positive	YS58	wt and ECM22 overexpressing strain were grown at 30.C in YPD medium
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C