S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gat1p	YKR039W	PubMed Stanbrough M et al., Proc Natl Acad Sci U S A, 1995 Oct 10;92(21):9450-4	Indirect	lacZ - wt vs TF mutant	Positive	S288c	cells grown on urea as nitrogen source
		PubMed Stanbrough M et al., J Bacteriol, 1995 Jan;177(1):94-102	Indirect	lacZ - wt vs TF mutant	Positive	S288c	urea
		PubMed Coffman JA et al., Mol Cell Biol, 1996 Mar;16(3):847-58	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Wickerham's minimal medium + proline
		PubMed Stanbrough M et al., J Bacteriol, 1996 Apr;178(8):2465-8	Indirect	lacZ - wt vs TF mutant	Positive	S288c	urea as N source
		PubMed Soussi-Boudekou S et al., Mol Microbiol, 1997 Mar;23(6):1157-68	Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Minimal medium + glutamine
			Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Minimal medium + NH4+
		PubMed Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	30 min 50 nM treatment with rapamycin in YPD
		PubMed Rubio-Texeira M, FEBS Lett, 2007 Feb 6;581(3):541-50	Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Cells grown at 24 .C in minimal medium with ammonia as nitrogen source
			Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Cells grown at 24 .C in minimal medium with glutamate as nitrogen source
			Indirect	lacZ - wt vs TF mutant	Positive	CKY835	Cells grown at 24 .C in minimal medium with glutamine as nitrogen source
		PubMed Georis I et al., Genetics, 2009 Mar;181(3):861-74	Direct	ChIP	N/A	TB123, FV018, FV036, FV041	YNB-glutamine medium treated with rapamycin (0.2 microg/ml) for 30 min
		PubMed Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source
		PubMed Fayyadkazan M et al., Microbiologyopen, 2014 Jun;3(3):271-87	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.
		PubMed Chen X et al., J Biotechnol, 2017;():Chen X et al., J Biotechnol, 2017;():	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YS58 strain with plasmid containing GAT1 ORF of MT2 or YS58 strains cloned into SalI and XbaI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	wt and GAT1 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	wt and GAT1 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
		PubMed Hong J et al., Elife, 2018 Apr 5;7(0):e32323	Indirect	GFP - wt vs TF mutant	Positive	FY4	Cells cultured in ammonium-limited chemostats
		PubMed Miller D et al., PLoS Genet, 2018 May 21;14(5):e1007406	Indirect	Barseq after FACS after mRNA FISH (BFF)	Positive	FY4	400 microM glutamine added to a culture of yeast cells growing in minimal media containing 800microM proline as a sole nitrogen source
			Indirect	Barseq after FACS after mRNA FISH (BFF)	Negative	FY4	culture of yeast cells growing in minimal media containing 800microM proline as a sole nitrogen source
		PubMed Wang YP et al., Bioengineering (Basel), 2021 May 8;8(5):	Indirect	RT-PCR - wt vs. TF mutant	Positive	S17	Cells cultured at 20 .C in the fermentation medium (12 .P wort) for 36 h