S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gat1p	YNL142W	PubMed Marini AM et al., Mol Cell Biol, 1997 Aug;17(8):4282-93	Indirect	lacZ - wt vs TF mutant	Negative	S288c	20mM Ammonium 0,1% glutamine(nitrogen source)
		PubMed Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	30 min 50 nM treatment with rapamycin in YPD
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Fayyadkazan M et al., Microbiologyopen, 2014 Jun;3(3):271-87	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.
		PubMed Hong J et al., Elife, 2018 Apr 5;7(0):e32323	Direct	EMSA	N/A	FY4	Cells cultured in ammonium-limited chemostats
			Indirect	RNA-seq - wt vs TF mutant	Negative	FY4	Cells cultured in ammonium-limited chemostats
			Indirect	GFP - wt vs TF mutant	Negative	FY4	Cells cultured in ammonium-limited chemostats
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Cells cultured in ammonium-limited chemostats containing ammonium
			Indirect	GFP - wt vs TF mutant	Positive	FY4	Cells cultured in ammonium-limited chemostats containing proline
			Indirect	GFP reporter (WT vs GAT1(R345G))	Negative	FY4	Cells cultured in ammonium-limited chemostats containing ammonium
			Indirect	GFP reporter (WT vs GAT1(R345G))	Negative	FY4	Cells cultured in ammonium-limited chemostats containing proline
			Indirect	GFP reporter (WT vs GAT1(W321L))	Negative	FY4	Cells cultured in ammonium-limited chemostats containing ammonium
			Indirect	GFP reporter (WT vs GAT1(W321L))	Negative	FY4	Cells cultured in ammonium-limited chemostats containing proline
			Indirect	GFP reporter (WT vs GAT1(C331Y))	Negative	FY4	Cells cultured in ammonium-limited chemostats containing ammonium
			Indirect	GFP reporter (WT vs GAT1(C331Y))	Negative	FY4	Cells cultured in ammonium-limited chemostats containing proline