Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Gcn4p
|
YBL033C
|
PubMed
Natarajan K et al., Mol Cell Biol, 2001 Jul;21(13):4347-68Natarajan K et al., Mol Cell Biol, 2001 Jul;21(13):4347-68 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
SC medium + 100mM 3-aminotriazole |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007Mascarenhas C et al., Mol Biol Cell, 2008 Jul;19(7):2995-3007 |
Indirect |
Microarray analysis - wt vs TF mutant |
Negative |
S288c |
minimalSDmedia+0.5mM H2O2 for 15min |
Indirect |
Microarray analysis - wt vs TF mutant |
Negative |
S288c |
minimalSDmedia+3-aminotriazole |
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
BY4741 |
YNB media |
PubMed
McIsaac RS et al., Nucleic Acids Res, 2013 Feb 1;41(4):e57McIsaac RS et al., Nucleic Acids Res, 2013 Feb 1;41(4):e57 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
S288c |
YPD medium; mid-log phase |
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311 |
Direct |
ChIP-seq |
N/A |
BY4741 |
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis |
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |