S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gcn4p	YCL030C	PubMed Li W et al., J Bacteriol, 1992 Jun;174(12):4148-56	Indirect	lacZ - wt vs TF mutant	Positive	S288c	3-aminotriazole
		PubMed Mountain HA et al., Yeast, 1991 Nov;7(8):781-803	Indirect	Northern blot - wt vs TF mutant	Positive	S288C	Minimal medium + methionine
		PubMed Arndt K et al., Proc Natl Acad Sci U S A, 1986;():	Direct	DNA footprinting	N/A	S288c	in vitro
		PubMed Lucchini G et al., Mol Cell Biol, 1984 Jul;4(7):1326-33	Indirect	lacZ - wt vs TF mutant	Positive	S288c	log phase cells grown in SD medium supplemented with 10 mM 3-aminotriazole
		PubMed Hinnebusch AG et al., Proc Natl Acad Sci U S A, 1983 Sep;80(17):5374-8	N/A		N/A	S288c	N/A
		PubMed O'Connell KF et al., Mol Cell Biol, 1995;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Yeast cells were grown in SCM lacking aromatic amino acids. From each culture, an initial sample was removed and the remaining portion was adjusted to 1 mM 5-MT, after which additional samples were removed at the times indicated (0, 1h, 2h and 4h)
		PubMed Mountain HA et al., Mol Microbiol, 1993 Jan;7(2):215-28	Indirect	Northern blot - wt vs TF mutant	Positive	F384	SC medium + aminotriazole
		PubMed Dudley AM et al., Genetics, 1999 Apr;151(4):1365-78	Direct	lacZ - wt vs mutant promoter sequence	Positive	S288c	SD medium; mid-log phase
		PubMed Natarajan K et al., Mol Cell Biol, 2001 Jul;21(13):4347-68	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	SC medium + 100mM 3-aminotriazole
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Yin Z et al., Proteomics, 2004 Aug;4(8):2425-36	Indirect	Proteomics - wt vs TF mutant	Positive	S288c	SC-HIS medium + 3-aminotriazole
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	SC medium; mid-log phase
		PubMed Kleinschmidt M et al., Mol Genet Genomics, 2005 Jun;273(5):382-93	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	3-aminotriazole
		PubMed Conesa C et al., Mol Cell Biol, 2005 Oct;25(19):8631-42	Indirect	Microarray analysis - wt vs TF mutant	Positive	YNN282	Constitutive derepression conditions;
		PubMed Kim SJ et al., Mol Cell Biol, 2005 Dec;25(24):11171-83	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	exposure to sulfometuron methyl
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Ernst J et al., Mol Syst Biol, 2007;():Ernst J et al., Mol Syst Biol, 2007;():	Direct	Chip-on-chip	N/A	S288c	Methylmethane sulfonate stress
		PubMed Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YNB media
		PubMed Joo YJ et al., Eukaryot Cell, 2009 Aug;8(8):1268-77	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	SC medium w/o histidine
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 10 mM 3-AT
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
		PubMed Herbig E et al., Mol Cell Biol, 2010 May;30(10):2376-90	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	sulfometuron methyl
		PubMed Fendt SM et al., Mol Syst Biol, 2010;():	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Knutson BA et al., Mol Cell Biol, 2011 Feb;31(4):818-31	Direct	ChIP	N/A	S288c	Galactose
		PubMed Joo YJ et al., EMBO J, 2011 Mar 2;30(5):859-72	Direct	ChIP	N/A	S288c	3-aminotriazole
		PubMed HernÃ¡ndez H et al., Biochem Biophys Res Commun, 2011 Jan 21;404(3):859-64	Direct	ChIP	N/A	S288c	3-aminotriazole
		PubMed Uluisik I et al., PLoS One, 2011;6(11):e27772	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	20 mM boric acid for 1h
		PubMed Brzovic PS et al., Mol Cell, 2011 Dec 23;44(6):942-53	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	sulfometuron methyl
		PubMed McIsaac RS et al., Nucleic Acids Res, 2013 Feb 1;41(4):e57	Indirect	Microarray analysis - wt vs TF overexpression	Positive	S288c	YPD medium; mid-log phase
		PubMed Lee JC et al., FEMS Yeast Res, 2013 Nov;13(7):618-34	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Cells grown to mid-exponential phase in SD min; addition of 100 mM L-serine
		PubMed Qiu H et al., Genome Res, 2016 Feb;26(2):211-25	Direct	ChIP	N/A	S288c	CellsÂ grown in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600Â = 0.6â€“0.8), and SM was added at 1 microg/mL for 30 min to induce Gcn4.Â
		PubMed Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457	Direct	ChIP-seq	N/A	BY4741	Cells grown on YPD and collected in mid-log phase at OD600 of 0.4-0.8
		PubMed GonzÃ¡lez J et al., Genetics, 2017 Nov;207(3):975-991	Direct	ChIP	N/A	CLA11-700	Cells grown to OD600 0.5 on MM with 2% glucose with Glutamine (biosynthetic conditions)
			Direct	ChIP	N/A	CLA11-700	Cells grown to OD600 0.5 on MM with 2% glucose with VIL (valine plus isoleucine plus leucine) (catabolic conditions)
			Direct	ChIP	N/A	CLA11-700	Cells grown to OD600 0.5 on MM with 2% glucose with GABA
			Indirect	Northern blot - wt vs TF mutant	Positive	CLA11-700	MM medium with 2% glucose with glutamine (7 mM)
		PubMed Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311	Direct	ChIP-seq	N/A	BY4741	Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
		PubMed Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
		PubMed Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252	Direct	ChIP	N/A	CEN.PK	Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)
			Indirect	RNA-seq - wt vs TF mutant	Positive	CEN.PK	Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C