PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
SC medium; mid-log phase
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Positive
BY4741
YNB media
PubMed
Fendt SM et al., Mol Syst Biol, 2010;():Fendt SM et al., Mol Syst Biol, 2010;():
Indirect
Microarray analysis - wt vs TF mutant
Negative
S288c
YPD medium; mid-log phase
PubMed
Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457Mittal N et al., Nat Commun, 2017 Sep 6;8(1):457
Direct
ChIP-seq
N/A
BY4741
Cells grown on YPD and collected in mid-log phase at OD600 of 0.4-0.8
PubMed
Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311
Direct
ChIP-seq
N/A
BY4741
Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
PubMed
Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252
Direct
ChIP
N/A
CEN.PK
Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)