S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gcn4p	YIL117C	PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Ernst J et al., Mol Syst Biol, 2007;():Ernst J et al., Mol Syst Biol, 2007;():	Direct	Chip-on-chip	N/A	S288c	Methylmethane sulfonate stress
		PubMed Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YNB media
		PubMed Fendt SM et al., Mol Syst Biol, 2010;():	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YPD medium; mid-log phase
		PubMed Rawal Y et al., Mol Cell, 2018 Apr 19;70(2):297-311	Direct	ChIP-seq	N/A	BY4741	Cells cultured in synthetic complete medium lacking isoleucine and valine (SC-Ilv) to log-phase (OD600=0.6-0.8) supplemented with SM at 1 microg/mL for 25 min to induce Gcn4 synthesis
		PubMed Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200	Indirect	RNA-seq - wt vs TF mutant	Negative	CEN.PK	RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation
		PubMed Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
		PubMed Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252	Indirect	RNA-seq - wt vs TF mutant	Negative	CEN.PK	Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)