PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Negative
BY4741
YNB media
PubMed
Godinho CP et al., Appl Microbiol Biotechnol, 2017 Jun;101(12):5005-5018Godinho CP et al., Appl Microbiol Biotechnol, 2017 Jun;101(12):5005-5018
Indirect
RT-PCR - wt vs TF mutant
Positive
BY4741
Cells grown on MM4 medium at pH 4.0 supplemented with benzoic acid (0.9 mM)
PubMed
Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200Walvekar AS et al., Mol Biol Cell, 2018 Oct 24;29(26):3183-3200
Indirect
RNA-seq - wt vs TF mutant
Negative
CEN.PK
RM (1% yeast extract, 2% peptone, and 2% lactate) medium grown cells shifted to MM (0.17% yeast nitrogen base without amino acids, 0.5% ammonium sulfate, and 2% lactate) medium with methionine supplementation
PubMed
Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252Srinivasan R et al., PLoS Genet, 2020 Dec;16(12):e1009252
Direct
ChIP
N/A
CEN.PK
Cells cultured for 1h in MM medium (Yeast Nitrogen Base with glucose as a carbon source) + Met (2mM methionine)
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314
