S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gln3p	YDL170W	PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	SC medium; mid-log phase
		PubMed Ishida C et al., Mol Microbiol, 2006 Mar;59(6):1790-806	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	3-aminotriazole
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	PUGA3-lacZ reporter plasmid was introduced into wt cells or into cells deleted for GCN2 and GLN3. Cells were cultured in synthetic complete medium supplemented with all amino acids except histidine, treated with 3-AT or rapamycin(Rap) for 6h
			Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	PUGA3-lacZ reporter plasmid was introduced into wt cells or into cells deleted for GCN4 and GLN3. Cells were cultured in synthetic complete medium supplemented with all amino acids except histidine, treated with 3-AT or rapamycin(Rap) for 6h
			Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	PUGA3-lacZ reporter plasmid was introduced into wt cells or into cells deleted for GLN3. Cells were cultured in synthetic complete medium supplemented with all amino acids except histidine, treated with rapamycin(Rap) for 6h
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 10 mM 3-AT
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 10 mM 3-AT
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
		PubMed Ponomarova O et al., Cell Syst, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq - wt vs TF mutant	Positive	S90	wt and DELTAgln3 cells were grown in YPAD medium
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C