Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Gln3p
|
YER040W
|
PubMed
Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 10 mM 3-AT |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 10 mM 3-AT |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgln3 cells treated for 1 h with 10 mM 3-AT |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
EG328-1A |
RNA prepared from wt and DELTAgln3 cells treated for 1 h with 200 nM Rapamycin |
PubMed
Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
YPD medium; mid-log phase |
PubMed
Chen X et al., J Biotechnol, 2017;():Chen X et al., J Biotechnol, 2017;(): |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
YS58 strain with plasmid containing GLN3 ORF of MT2 or YS58 strains cloned into SalI and SacI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
YS58 |
wt and GLN3 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours, in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source. |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
YS58 |
wt and GLN3 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source. |