PubMed
Talibi D et al., Nucleic Acids Res, 1995 Feb 25;23(4):550-7Talibi D et al., Nucleic Acids Res, 1995 Feb 25;23(4):550-7
Indirect
lacZ - wt vs TF mutant
Positive
S288c
minimal proline medium (0,1% proline) + 0,1% GABA
PubMed
Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
30 min 50 nM treatment with rapamycin in YPD
PubMed
Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911
Indirect
lacZ - wt vs TF mutant
Negative
EG328-1A
yeast cells deleted for GCN4 and GLN3 were transformed with a plasmid encoding a lacZ reporter gene fused to the UGA1 promoter. Cells were cultured in synthetic medium containing ammonia as the nitrogen source and then switched to synthetic medium containing 10 mM GABA as the nitrogen source for 6 h
Indirect
lacZ - wt vs TF mutant
Negative
EG328-1A
yeast cells deleted for GLN3 were transformed with a plasmid encoding a lacZ reporter gene fused to the UGA1 promoter. Cells were cultured in synthetic medium containing ammonia as the nitrogen source and then switched to synthetic medium containing 10 mM GABA as the nitrogen source for 6 h
PubMed
Cardillo SB et al., Microbiology, 2012;():Cardillo SB et al., Microbiology, 2012;():