S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gln3p	YHL016C	PubMed ElBerry HM et al., J Bacteriol, 1993 Aug;175(15):4688-98	Indirect	Northern blot - wt vs TF mutant	Positive	SIGMA1278b	Wickerham's minimal medium + proline
		PubMed Cox KH et al., Yeast, 1999 Jun 15;15(8):703-13	Indirect	Microarray analysis - wt vs TF mutant	Negative	TCY1	Wickerham's minimal medium with 0,6% glucose and 0,1% of proline
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	SC medium; mid-log phase
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
		PubMed Zhao X et al., Appl Environ Microbiol, 2014 Jan;80(1):392-8	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Gln3p with S344A S347A S355A mutations or/and Gln3p with truncation of the exact C-terminal NLRS YNB medium supplemented with glutamine (10 mmol/liter). wt S. cerevisiae CEN PK2 strain was used as the control.
		PubMed Ponomarova O et al., Cell Syst, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq - wt vs TF mutant	Negative	S90	wt and DELTAgln3 cells were grown in YPAD medium
		PubMed Zhang P et al., J Agric Food Chem, 2018 Oct 17;66(41):10881-10889	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	DELTAgzf3 vs DELTAgzf3+overIndirect of GLN3 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs wt-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAgzf3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAgln3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAgat1-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	wt vs DELTAdal80-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.