S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Haa1p	YGR138C	PubMed Keller G et al., J Biol Chem, 2001 Oct 19;276(42):38697-702	Direct	DNA footprinting	Positive	CM66J	Cells grown in SC medium before induction of pGAL1-HAA1 with 2% galactose
			Direct	DNA footprinting	Positive	CM66J	Cells grown to early log phase in medium containing 10 microM CuSO4 or 100 microM BCS before induction of pGAL1-HAA1 with 2% galactose
			Direct	DNA footprinting	Positive	CM66J	Cells grown to log phase in SC medium
			Direct	DNA footprinting	Positive	CM66J	Expression of Haa1(1-124)/VP16 fusion induced in cells growing in medium containing 100 microM CuSO4 or 100 microM BCS with 2% galactose
			Indirect	Microarray analysis - wt vs TF mutant	Positive	CM66J	Cells grown to log phase in SC medium supplemented with 100 microM BCS or 100 microM CuSO4
		PubMed Aranda A et al., Appl Environ Microbiol, 2004 Apr;70(4):1913-22	Indirect	Western blot - wt vs TF mutant	Positive	S288c	1 g/L acetaldehyde (1H)
		PubMed Fernandes AR et al., Biochem Biophys Res Commun, 2005 Nov 11;337(1):95-103	Indirect	Western blot - wt vs TF mutant	Positive	S288c	20 mM propionic acid (45 min)
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Mira NP et al., OMICS, 2010 Oct;14(5):587-601	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Cells cultivated for 30 min in MM4 growth medium (pH 4.0) supplemented with 50 mM acetic acid
		PubMed Mira NP et al., OMICS, 2010 Oct;14(5):587-601	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	Cells cultivated for 30 min in MM4 growth medium (pH 4.0) supplemented with 50 mM acetic acid
		PubMed Tanaka K et al., Appl Environ Microbiol, 2012 Nov;78(22):8161-3	Indirect	RT-PCR - wt vs TF overexpression (altered promoter sequence)	Positive	S288c	logarithmically growing cells
		PubMed Sakihama Y et al., J Biosci Bioeng, 2015 Mar;119(3):297-302	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	acetic acid
		PubMed Baek SH et al., Appl Microbiol Biotechnol, 2016 Mar;100(6):2737-48	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	Control
		PubMed Swinnen S et al., Microb Cell Fact, 2017 Jan 9;16(1):7	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Growth in synthetic medium + 50 mM acetic acid (pH 4.5)
		PubMed Collins ME et al., Appl Environ Microbiol, 2017 Jun 16;83(13):e00672-17	Indirect	lacZ - wt vs TF mutant	Positive	S288c	log-phase growth; MM4 pH 4.0
			Indirect	lacZ - wt vs TF mutant	Positive	S288c	60 mM acetic acid (6 generations)
		PubMed Kim MS et al., Nucleic Acids Res, 2019 Feb 20;47(3):1211-1224	Direct	ChIP	N/A	S288c	Weak acid stress
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	30 mM acetic acid (30 min)
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	300 mM lactic acid (30 min)
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	20 mM propionic acid (30 min)
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	10 mM sorbic acid (30 min)
			Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	2 mM benzoic acid (30 min)