PubMed
Repetto B et al., Mol Cell Biol, 1989 Jun;9(6):2695-705Repetto B et al., Mol Cell Biol, 1989 Jun;9(6):2695-705
Indirect
lacZ - wt vs TF mutant
Positive
D273-10B/A1
WOGal medium (2% galactose, 0.67% Difco yeast nitrogen base without amino acids)
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000
Direct
ChIP-exo
N/A
CEN.PK 113-5D
Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h