S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Hap4p	YJR048W	PubMed Olesen J et al., Cell, 1987 Dec 24;51(6):953-61	Direct	EMSA	N/A	S288c	N/A
		PubMed Daignan-Fornier B et al., J Biol Chem, 1994 Jun 3;269(22):15469-72	Indirect	lacZ - wt vs TF mutant	Positive	BWG1-7a	Minimal medium with galactose
		PubMed Blom J et al., Appl Environ Microbiol, 2000 May;66(5):1970-3	Indirect	Northern blot - wt vs TF overexpression	Positive	S288c	YPD medium; mid-log phase
		PubMed Ramil E et al., Mol Microbiol, 2000 Sep;37(5):1116-32	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Minimal medium with 0,1% glucose 2% ethanol 2% lactate
		PubMed Raghevendran V et al., J Biol Chem, 2006 May 5;281(18):12308-14	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	YP medium; respiratory metabolism (chemostat under glucose limitation)
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Schuurmans JM et al., FEMS Yeast Res, 2008 Feb;8(1):26-34	Indirect	Microarray analysis - wt vs TF mutant	Positive	CEN.PK113-7D	wt versus Tf overexpression 0.67 %YNB, 2% glucose and 0.1 M phtalic acid pH 5 30.C mid exp cells
		PubMed Morohashi N et al., Biol Pharm Bull, 2008 Feb;31(2):187-92	Direct	In vivo UV-photofootprinting	N/A	S288c	SC medium; mid-log phase
		PubMed Ihrig J et al., Eukaryot Cell, 2010 Mar;9(3):460-71	Indirect	Luciferase - wt vs mutant promoter sequence	Positive	S288c	50mM FeCl3
		PubMed Raab AM et al., Appl Environ Microbiol, 2011 Mar;77(6):1981-9	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	Yeast cells were cultured in WMVIII medium with 5% (wt/vol) glucose supplemented with histidine (100 mg literâˆ’1) on a rotary shaker (150 rpm)
		PubMed Barba-Aliaga M et al., Int J Mol Sci, 2020 Dec 28;22(1):	Indirect	RT-PCR - wt vs. TF mutant	Positive	BY4741	SC medium 7 h