S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Hsf1p	YBR072W	PubMed Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	early response to heat shock (23 to 39ºC); low activity allele
		PubMed Treger JM et al., J Biol Chem, 1998 Oct 9;273(41):26875-9	Indirect	Northern blot - wt vs TF mutant	Positive	JT659	Heat-shock treatment
		PubMed Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83	Indirect	Proteomics - wt vs TF mutant	Positive	MYY290	Heat-shock: Exponential-phase cells shifted from 25C to 38C
		PubMed AmorÃ³s M et al., Mol Microbiol, 2001 Mar;39(6):1523-32	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Carbon source starvation
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Heat shock
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Osmotic stress
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	oxidative stress
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium 39ºC (heat shock)
		PubMed Imazu H et al., Eukaryot Cell, 2005 Jun;4(6):1050-6	Indirect	RT-PCR - wt vs TF mutant	Positive	HS126	Cells were grown in ESD medium lacking uracil at 28.C, and then the temperature was shifted to 39.C.
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min)
		PubMed Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182	Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine), 5Â´heat shock and 120Â´heat shock
			Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine), 5' heat shock
			Indirect	NAC-seq (nascent mRNA-sequencing)	Positive	BY4741	YPDA (with 0.002% adenine), 5' heat shock
		PubMed Vallabhaneni AR et al., Curr Genet, 2021 Dec;67(6):937-951	Direct	ChIP	N/A	W3031	SC media