S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Hsf1p	YLL026W	PubMed Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7	Indirect	Western blot - wt vs TF mutant	Positive	S288c	growth at 34ºC; ?ssa1/2 background with low activity allele
		PubMed Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83	Indirect	Proteomics - wt vs TF mutant	Positive	MYY290	Heat-shock: Exponential-phase cells shifted from 25C to 38C
		PubMed AmorÃ³s M et al., Mol Microbiol, 2001 Mar;39(6):1523-32	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Carbon source starvation
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Heat shock
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Osmotic stress
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	oxidative stress
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium 39ºC (heat shock)
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min)
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed SolÃs EJ et al., Mol Cell, 2016 Jul 7;63(1):60-71	Indirect	Net-seq	Positive	S288c	basal conditions
		PubMed de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290	Direct	ChIP	N/A	S288C	SC medium
		PubMed de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290	Indirect	RT-PCR - wt vs TF mutant	Negative	S288C	SC medium
		PubMed Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182	Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine), non-heat shock, 5Â´heat shock and 120Â´heat shock
			Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock
			Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine), 5' heat shock
			Indirect	NAC-seq (nascent mRNA-sequencing)	Positive	BY4741	YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock
			Indirect	NAC-seq (nascent mRNA-sequencing)	Positive	BY4741	YPDA (with 0.002% adenine), 5' heat shock
		PubMed Chowdhary S et al., Cell Rep, 2019 Jan 2;26(1):18-28.e5	Direct	ChIP	N/A	BY4741	YPDA
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C