Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Ino2p
|
YJL153C
|
PubMed
Hirsch JP et al., Mol Cell Biol, 1986 Oct;6(10):3320-8Hirsch JP et al., Mol Cell Biol, 1986 Oct;6(10):3320-8 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
cells grown in 10 mM inositol; synthetic medium |
PubMed
Donahue TF et al., J Biol Chem, 1981 Jul 10;256(13):7077-85Donahue TF et al., J Biol Chem, 1981 Jul 10;256(13):7077-85 |
N/A |
|
N/A |
S288c |
N/A |
PubMed
Nikoloff DM et al., J Biol Chem, 1994 Mar 11;269(10):7402-11Nikoloff DM et al., J Biol Chem, 1994 Mar 11;269(10):7402-11 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
BRS 1127 |
SC medium |
Indirect |
lacZ - wt vs TF mutant |
Positive |
BRS 1127 |
SC medium without inositol |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
Hoppen J et al., Yeast, 2005 Jun;22(8):601-13Hoppen J et al., Yeast, 2005 Jun;22(8):601-13 |
Indirect |
lacZ - wt vs TF mutant |
Positive |
SS92.3-1 |
Depressing conditions: 5 microM . inositol +5 microM choline |
Indirect |
lacZ - wt vs TF mutant |
Positive |
SS92.3-1 |
Repressing conditions: 200 microM inositol +2mM choline |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
El Kaderi B et al., J Biol Chem, 2009 Sep 11;284(37):25015-25El Kaderi B et al., J Biol Chem, 2009 Sep 11;284(37):25015-25 |
Direct |
ChIP |
N/A |
S288c |
N/A |
PubMed
Esposito M et al., Biochem Biophys Res Commun, 2010 Jan 8;391(2):1285-90Esposito M et al., Biochem Biophys Res Commun, 2010 Jan 8;391(2):1285-90 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
SC medium; mid-log phase |
PubMed
Shetty A et al., Eukaryot Cell, 2010 Dec;9(12):1845-55Shetty A et al., Eukaryot Cell, 2010 Dec;9(12):1845-55 |
Direct |
ChIP |
N/A |
S288c |
Without Inositol and Cholin |
PubMed
Wimalarathna R et al., J Microbiol, 2011 Apr;49(2):265-73Wimalarathna R et al., J Microbiol, 2011 Apr;49(2):265-73 |
Indirect |
RT-PCR - wt vs TF mutant |
Negative |
S288c |
exposure to 100 µM inositol |
PubMed
Gururaj C et al., J Biol Chem, 2013 Jul 12;288(28):20453-63Gururaj C et al., J Biol Chem, 2013 Jul 12;288(28):20453-63 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Inositol depletion |
PubMed
Chumnanpuen P et al., BMC Syst Biol, 2013;():Chumnanpuen P et al., BMC Syst Biol, 2013;(): |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
Steady-state aerobic chemostat 30.C |
PubMed
Bergenholm D et al., mSystems, 2018 Jul-Aug;3(4):Bergenholm D et al., mSystems, 2018 Jul-Aug;3(4): |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Steady state aerobic chemostat cultivation at 30 .C in nitrogen-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Steady state aerobic chemostat cultivation at 30 .C in glucose-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Steady state chemostat cultivation at 30 .C in oxygen- and glucose-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Steady state aerobic chemostat cultivation at 30 .C in ethanol-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0 |
PubMed
Ravi C et al., Curr Genet, 2019 Aug;65(4):995-1014Ravi C et al., Curr Genet, 2019 Aug;65(4):995-1014 |
Indirect |
BETA-galactosidase assay |
Positive |
BY4741 |
synthetic complete medium-Ura 30 .C for 4 h |
Indirect |
BETA-galactosidase assay |
Positive |
BY4741 |
synthetic complete medium-Ura with 75 microM inositol, 30 .C for 4 h |
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314 |
Direct |
ChIP-exo |
N/A |
BY4741 |
YPD medium, 25.C |