S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Leu3p	YCL018W	PubMed Brisco PR et al., J Biol Chem, 1990 Jul 15;265(20):11667-75	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	SD medium
		PubMed Friden P et al., Mol Cell Biol, 1987 Aug;7(8):2708-17	Direct	EMSA	N/A	S288c	in vitro
		PubMed Friden P et al., Mol Cell Biol, 1988 Jul;8(7):2690-7	Indirect	lacZ - wt vs TF mutant	Positive	S288c	SD medium
		PubMed Brisco PR et al., Genetics, 1987 Jan;115(1):91-9	Indirect	Enzyme activity - wt vs TF mutant	Positive	S288c	leucine absence
		PubMed Hellauer K et al., Mol Cell Biol, 1996 Nov;16(11):6096-102	Indirect	lacZ - wt vs TF mutant	Positive	S288c	SC medium; mid-log phase cells
		PubMed NoÃ«l J et al., J Biol Chem, 1998 Jul 10;273(28):17463-8	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Strains were grown overnight in YPD. Cells were then diluted in SD supplemented with histidine, leucine, lysine, tryptophan, and adenine at 0.004% (w/v) and glucose (2%). Activity of Uga3p was induced by adding GABA to the medium (0.1% final concentration).
		PubMed Onda M et al., Gene, 2004;():Onda M et al., Gene, 2004;():	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPGal + Adenine
		PubMed Boer VM et al., FEMS Yeast Res, 2005 Jul;5(10):885-97	Indirect	Microarray analysis - wt vs TF mutant	Positive	CEN.PK540-3A	Ethanol-limitation: Defined synthetic medium (NH4)2SO4, 5.0 g/l;KH2PO4,3.0 g/l; MgSO4.7H2O, 0.5 g/l; ethanol, 6.0 g/l; vitamins and trace elements.
		PubMed Tang L et al., BMC Genomics, 2006;():Tang L et al., BMC Genomics, 2006;():	Indirect	Microarray analysis - wt vs TF overexpression	Positive	BY4741	The cells were cultured in uracil dropout medium (YNB-AA (Sigma) 6.7 g/L, 0.77 g/L, Ura DO Supp., and 2% glucose supplemented with G418 at 200 mg/L) with shaking at 30.C. The cells were harvested in log-phase growth (A600 ~ 0.7).
		PubMed Cardillo SB et al., Eukaryot Cell, 2010 Aug;9(8):1262-71	Direct	ChIP	N/A	S288c	extracellular leucine and GABA
		PubMed Chubukov V et al., Proc Natl Acad Sci U S A, 2012 Mar 27;109(13):5127-32	Indirect	GFP - wt vs TF mutant	Positive	S288c	leucine absence
		PubMed Börlin CS et al., FEMS Microbiol Lett, 2020 Jul 1;367(13):	Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 100 mg/L isoleucine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 100 mg/L leucine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 100 mg/L valine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 20 mg/L leucine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm.