PubMed
Cardillo SB et al., Eukaryot Cell, 2010 Aug;9(8):1262-71Cardillo SB et al., Eukaryot Cell, 2010 Aug;9(8):1262-71
Indirect
lacZ - wt vs TF mutant
Negative
S288c
extracellular leucine and GABA
PubMed
Cardillo SB et al., Biochem Biophys Res Commun, 2011 Jul 15;410(4):885-9Cardillo SB et al., Biochem Biophys Res Commun, 2011 Jul 15;410(4):885-9
Indirect
RT-PCR - wt vs TF mutant
Negative
S288c
Minimal Media + Leu + GABA
PubMed
Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701
Indirect
lacZ - wt vs TF mutant
Negative
23344c
Minimal medium containing a 0.17% Difco yeast nitrogen base (YNB without amino acids and ammonium sulphate), 2% glucose and 10mM proline.
Indirect
lacZ - wt vs TF mutant
Negative
23344c
Minimal medium (0.17% Difco yeast nitrogen base, 2% glucose and 10mM proline) after incubation with 0.1mM GABA during 1h .
Indirect
lacZ - wt vs TF mutant
Positive
23344c
wt and DELTAgln3DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min
Indirect
lacZ - wt vs TF mutant
Negative
23344c
wt and DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min
Indirect
lacZ - wt vs TF mutant
Negative
23344c
wt and DELTAdal80DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min
Indirect
lacZ - wt vs TF mutant
Negative
23344c
wt and DELTAgzf3DELTAdal80DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min
Indirect
lacZ - wt vs TF mutant
Positive
23344c
wt and DELTAgzf3DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min
Indirect
lacZ - wt vs TF mutant
Negative
23344c
wt and DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min