|
Regulation Reference(s)
The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Met31p
|
YLR303W
|
PubMed
Blaiseau PL et al., Mol Cell Biol, 1997 Jul;17(7):3640-8Blaiseau PL et al., Mol Cell Biol, 1997 Jul;17(7):3640-8 |
Indirect |
Northern blot - wt vs TF mutant |
Negative |
S288c |
medium without metionine for 60min |
PubMed
Aranda A et al., Appl Environ Microbiol, 2004 Apr;70(4):1913-22Aranda A et al., Appl Environ Microbiol, 2004 Apr;70(4):1913-22 |
Indirect |
Western blot - wt vs TF mutant |
Positive |
S288c |
1 g/L acetaldehyde (1H) |
Indirect |
Western blot - wt vs TF mutant |
Positive |
S288c |
log-phase growth; 2 % glucose YPD |
PubMed
Su NY et al., J Biol Chem, 2008 Apr 25;283(17):11615-24Su NY et al., J Biol Chem, 2008 Apr 25;283(17):11615-24 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
BF264-15D |
met31met32DELTA double deletion mutant vs met31DELTA single mutant SC media 30.C lacking methionine |
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
BY4741 |
YNB media |
PubMed
Lee TA et al., Mol Biol Cell, 2010 Feb 1;21(3):456-69Lee TA et al., Mol Biol Cell, 2010 Feb 1;21(3):456-69 |
Indirect |
Microarray analysis - wt vs TF mutant |
Negative |
W303 (met4::GAL-MET4 met30DELTA met31DELTA met32DELTA) |
Shift from YEP + 2% raffinose to YEP + 2-3% galactose |
PubMed
Carrillo E et al., Mol Biol Cell, 2012 May;23(10):1928-42Carrillo E et al., Mol Biol Cell, 2012 May;23(10):1928-42 |
Direct |
ChIP |
N/A |
W303 |
90 min of Met4 induction |
|
|
|