PubMed
Pereira Y et al., Toxicol Sci, 2008 Dec;106(2):400-12Pereira Y et al., Toxicol Sci, 2008 Dec;106(2):400-12
Indirect
Microarray analysis - wt vs TF mutant
Positive
YPH98
135 min at 30.C in YNB medium + 20microM chromate
PubMed
Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82
Indirect
Microarray analysis - wt vs TF mutant
Positive
BY4741
YNB media
PubMed
Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014
Direct
EMSA
N/A
in vitro
E. coli cell extracts expressing polyhistidine-tagged Met32 or not were incubated with equimolar amounts of the indicated 32P-labeled DNA fragments.
Direct
ChIP
N/A
W303
Isogenic strain expressing Myc-tagged Met32 was grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+. Binding to PDC6 upstream region (from -455 to -306 relative to ATG)
Direct
ChIP
N/A
W303
Isogenic strain expressing Myc-tagged version of Met32 was grown to early log phase in YPD medium and exposed to 0.5mM Cd2+.
Indirect
RT-PCR - wt vs TF mutant
Positive
W303
wt vs DELTAmet31DELTAmet32: cells were grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+
Indirect
RT-PCR - wt vs TF mutant
Positive
W303
wt vs DELTAmet32: cells were grown to early log phase in YPD medium and exposed to 0.5 mM Cd2+
PubMed
Carrillo E et al., Mol Biol Cell, 2012 May;23(10):1928-42Carrillo E et al., Mol Biol Cell, 2012 May;23(10):1928-42
Direct
Chip-on-chip
N/A
W303
90 min of Met4 induction
PubMed
Petti AA et al., Mol Biol Cell, 2012 Aug;23(15):3008-24Petti AA et al., Mol Biol Cell, 2012 Aug;23(15):3008-24