PubMed
Lambrechts MG et al., Proc Natl Acad Sci U S A, 1996 Aug 6;93(16):8419-24Lambrechts MG et al., Proc Natl Acad Sci U S A, 1996 Aug 6;93(16):8419-24
Indirect
Northern blot - wt vs TF mutant
Positive
S288c
SCSG medium (0.67% yeast nitrogen base without amino acids, 2% starch, 0.05% glucose and all required growth factors)
PubMed
Gagiano M et al., J Bacteriol, 1999 Oct;181(20):6497-508Gagiano M et al., J Bacteriol, 1999 Oct;181(20):6497-508
Indirect
lacZ - wt vs TF overexpression
Positive
S288c
SCGE (3% glycerol 3% ethanol) medium
PubMed
van Dyk D et al., Genetics, 2005 Jan;169(1):91-106van Dyk D et al., Genetics, 2005 Jan;169(1):91-106
Indirect
Northern blot - wt vs TF mutant
Positive
S288c
SCD medium
PubMed
Fichtner L et al., Mol Microbiol, 2007 Dec;66(5):1276-89Fichtner L et al., Mol Microbiol, 2007 Dec;66(5):1276-89
Indirect
RT-PCR - wt vs TF mutant
Negative
S288C
wt versus TF mutant Sc media OD:0.7
PubMed
Barrales RR et al., Genetics, 2008 Jan;178(1):145-56Barrales RR et al., Genetics, 2008 Jan;178(1):145-56
Indirect
Northern blot - wt vs TF mutant
Positive
S288c
YPD medium; mid-log phase
PubMed
Chavel CA et al., PLoS Genet, 2010 Mar 19;6(3):e1000883Chavel CA et al., PLoS Genet, 2010 Mar 19;6(3):e1000883
Indirect
RT-PCR - wt vs TF mutant
Negative
SIGMA1278b
wt vs DELTAmsn1: total RNA was isolated from 25 ml cultures grown in YEP GAL for 8h.