S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Msn2p	YCL040W	PubMed Boy-Marcotte E et al., J Bacteriol, 1998 Mar;180(5):1044-52	Indirect	Proteomics - wt vs TF mutant	Positive	W303	YNB medium
		PubMed Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83	Indirect	Proteomics - wt vs TF mutant	Positive	W303-A	Heat-shock: Exponential-phase cells shifted from 25C to 38C
		PubMed Herrero P et al., Biochem J, 1999;():Herrero P et al., Biochem J, 1999;():	Direct	EMSA	N/A	S288c	Yeasts were grown on 1% (w}v) yeast extract and 2% (w}v) peptone supplemented with 3% (v}v) ethanol (YEPE) at 28 °C until the OD600nm reached 1.0 (early log phase)
		PubMed Tadi D et al., Yeast, 1999 Dec;15(16):1733-45	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Diauxic shift; Late-exponential phase cells cultivated in YNB growth medium (0Â·17% yeast nitrogen base, 0Â·5% ammonium sulphate and 2% glucose)
		PubMed Rep M et al., J Biol Chem, 2000 Mar 24;275(12):8290-300	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD + 0,5M NaCl
		PubMed Hasan R et al., Mol Microbiol, 2002 Jul;45(1):233-41	Indirect	Proteomics - wt vs TF mutant	Positive	YPH98	Oxidative stess; cells grown in YNB medium to exponential phase exposed to 0.5 mM H2O2 for 30 min
		PubMed Hasan R et al., Mol Microbiol, 2002 Jul;45(1):233-41	Indirect	2D electrophoresis (WT vs msn2DELTAmsn4DELTAyap1DELTA triple mutant)	Positive	YPH98	Oxidative stess; cells grown in YNB medium to exponential phase exposed to 0.5 mM H2O2 for 30 min
		PubMed Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min)
		PubMed Berry DB et al., Mol Biol Cell, 2008 Nov;19(11):4580-7	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD+0.5 mM H2O2
			Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD+0.7 M NaCl 45min
		PubMed Capaldi AP et al., Nat Genet, 2008;():Capaldi AP et al., Nat Genet, 2008;():	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium containing 0,8M glucose (glucose "osmotic" stress)
		PubMed Hazelwood LA et al., Appl Environ Microbiol, 2009 Nov;75(21):6876-85	Indirect	Microarray analysis - wt vs TF mutant	Positive	IMZ066	Carbon-limiting (25 g/L glucose, 5 g/L (NH4)2SO4, 3 KH2PO4, 0.5 g/L MgSO4.7H2O, 0.0045 g/L ZnSO4.7H2O)
		PubMed Casado C et al., Biochem J, 2011 Sep 15;438(3):523-33	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	10 min shift to pH=8
		PubMed Stewart-Ornstein J et al., Curr Biol, 2013 Dec 2;23(23):2336-45	Indirect	GFP - wt vs TF overexpression	Positive	S288c	Estradiol induced
		PubMed Elfving N et al., Nucleic Acids Res, 2014 May;42(9):5468-82	Indirect	Microarray analysis - wt vs TF overexpression	Positive	S288c	SD + glucose + estradiol induction
		PubMed Kuang Z et al., Elife, 2017 Sep 26;6(0):e29938	Direct	ChIP-seq	N/A	BY5764	YPD 30.C