S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Msn2p	YFL014W	PubMed MartÃnez-Pastor MT et al., EMBO J, 1996 May 1;15(9):2227-35	Indirect	lacZ - wt vs TF mutant	Positive	S288c	7% ethanol 20min
		PubMed Schmitt AP et al., Proc Natl Acad Sci U S A, 1996 Jun 11;93(12):5777-82	Indirect	Northern blot - wt vs TF overexpression	Positive	S288c	Methylmethane sulfonate stress
		PubMed Smith A et al., EMBO J, 1998 Jul 1;17(13):3556-64	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	SC medium; mid-log phase
		PubMed Treger JM et al., J Biol Chem, 1998 Oct 9;273(41):26875-9	Indirect	Northern blot - wt vs TF mutant	Positive	JT659	Heat-shock treatment
		PubMed Rep M et al., Mol Cell Biol, 1999 Aug;19(8):5474-85	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	0,7M NaCl 60min
		PubMed Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83	Indirect	Proteomics - wt vs TF mutant	Positive	W303-A	Heat-shock: Exponential-phase cells shifted from 25C to 38C
		PubMed Causton HC et al., Mol Biol Cell, 2001 Feb;12(2):323-37	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium + 0,05M succinic acid, pH 4,0
		PubMed AmorÃ³s M et al., Mol Microbiol, 2001 Mar;39(6):1523-32	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Carbon source starvation
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Heat shock
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Osmotic stress
			Indirect	Northern blot - wt vs TF mutant	Positive	S288c	oxidative stress
		PubMed Hasan R et al., Mol Microbiol, 2002 Jul;45(1):233-41	Indirect	Proteomics - wt vs TF mutant	Positive	YPH98	Oxidative stess; cells grown in YNB medium to exponential phase exposed to 0.5 mM H2O2 for 30 min
		PubMed Hasan R et al., Mol Microbiol, 2002 Jul;45(1):233-41	Indirect	2D electrophoresis (WT vs msn2DELTAmsn4DELTAyap1DELTA triple mutant)	Positive	YPH98	Oxidative stess; cells grown in YNB medium to exponential phase exposed to 0.5 mM H2O2 for 30 min
		PubMed Kandror O et al., Mol Cell, 2004 Mar 26;13(6):771-81	Indirect	Northern blot - wt vs TF mutant	N/A	S288c	N/A
		PubMed Lai LC et al., Mol Cell Biol, 2005 May;25(10):4075-91	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	SSGal-TEA medium + anaerobiosis (2 generations)
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min)
		PubMed Erkina TY et al., Mol Cell Biol, 2008 Feb;28(4):1207-17	Direct	ChIP	N/A	S288c	YPD medium; mid-log phase
		PubMed Melamed D et al., RNA, 2008 Jul;14(7):1337-51	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Osmotic stress; Exponential phase cells cultivated in YPD growth medium supplemented with1 M NaCl
		PubMed Berry DB et al., Mol Biol Cell, 2008 Nov;19(11):4580-7	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD+0.7 M NaCl 45min
		PubMed Capaldi AP et al., Nat Genet, 2008;():Capaldi AP et al., Nat Genet, 2008;():	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium containing 0,8M glucose (glucose "osmotic" stress)
		PubMed Ni L et al., Genes Dev, 2009 Jun 1;23(11):1351-63	Direct	Chip-on-chip	N/A	S288c	SD medium + NaCl 0,6M 30'
		PubMed Kelley R et al., PLoS Genet, 2009 May;5(5):e1000488	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD 30.C from 0.1 mM H2O2 to 0.4 mM H2O2
		PubMed Hazelwood LA et al., Appl Environ Microbiol, 2009 Nov;75(21):6876-85	Indirect	Microarray analysis - wt vs TF mutant	Positive	IMZ066	Nitrogen-limiting (45 g/L glucose, 1 g/L (NH4)2SO4, 5.3 g/L K2SO4, 3 KH2PO4, 0.5 g/L MgSO4.7H2O, 0.0045 g/L ZnSO4.7H2O)
		PubMed Watanabe D et al., Appl Environ Microbiol, 2011 Feb;77(3):934-41	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Sake mash on day 5
		PubMed Kim HS et al., Appl Microbiol Biotechnol, 2011 Aug;91(4):1159-72	Indirect	Northern blot - wt vs TF mutant	Negative	S288c	YPD+10%ethanol, 30 min
		PubMed Sadeh A et al., Mol Biol Cell, 2011 Sep;22(17):3127-38	Indirect	GFP - wt vs TF mutant	Positive	S288c	SC medium; mid-log phase
		PubMed Huebert DJ et al., Mol Cell Biol, 2012 May;32(9):1645-53	Direct	Chip-on-chip	N/A	S288c	H2O2 treatment
		PubMed Stewart-Ornstein J et al., Curr Biol, 2013 Dec 2;23(23):2336-45	Indirect	GFP - wt vs TF overexpression	Positive	S288c	Estradiol induced
		PubMed Elfving N et al., Nucleic Acids Res, 2014 May;42(9):5468-82	Indirect	Microarray analysis - wt vs TF overexpression	Positive	S288c	SD + glucose + estradiol induction
		PubMed Gutin J et al., Mol Syst Biol, 2015 Oct 7;11(10):829	Indirect	GFP - wt vs TF mutant	Positive	S288c	Log phase
		PubMed Cho BR et al., Biochim Biophys Acta Gene Regul Mech, 2017 Jun;1860(6):695-704	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	1Â mM H2O2
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	2% lactic acid
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	2% glucose
		PubMed Rajvanshi PK et al., J Biol Chem, 2017 Nov 10;292(45):18628-18643	Direct	ChIP	N/A	BY4741	2% or 6% glucose, stationary phase
		PubMed Kuang Z et al., Elife, 2017 Sep 26;6(0):e29938	Direct	ChIP-seq	N/A	BY5764	YPD 30.C
		PubMed Bai C et al., PLoS One, 2020;15(8):e0237540	Indirect	BETA-galactosidase activity WT vs. TF double mutant + plasmid with promoter sequence	Positive	BY4741	YNB (-ura) + 0.7M NaCl (1 hour after exposure)
			Indirect	BETA-galactosidase activity WT vs. TF quadruple mutant + plasmid with promoter sequence	Positive	SP1	YNB (-ura) + 0.7M NaCl (1 hour after exposure)
			Indirect	BETA-galactosidase activity WT vs. TF triple mutant + plasmid with promoter sequence	Positive	BY4741	YNB (-ura) + 0.7M NaCl (1 hour after exposure)
			Indirect	RT-PCR - wt vs. TF double mutant	Positive	BY4741	YPD + 0,7M NaCl (1 hour after exposure)
			Indirect	RT-PCR - wt vs. TF quadruple mutant	Positive	SP1	YPD + 0,7M NaCl (1 hour after exposure)
		PubMed , , ;():, , ;():	Indirect	flow cytometry - GFP fluorescence	Positive	W303	exposed to different QSMs for 45 min (quorum-sensing molecule), Selective Complete medium (SC, -His)