S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Oaf1p	YIL160C	PubMed Karpichev IV et al., Mol Cell Biol, 1997 Jan;17(1):69-80	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	cells grown in YP Glycerol medium with oleic acid (induction conditions)
		PubMed Karpichev IV et al., Mol Cell Biol, 1998 Nov;18(11):6560-70	Indirect	Northern blot - wt vs TF mutant	Positive	W3031A	YP-Glycerol-Oleate
		PubMed Rottensteiner H et al., Biochem J, 2002;():	Direct	EMSA	N/A	BJ1991	Cells grown in YPD (0.05% glucose) medium with 0.2% oleic acid for 18 h
			Indirect	Western blot - wt vs TF mutant	Positive	BJ1991	Cells grown in fatty acid medium with 0.01% oleic acid or 0.001% lauric acid for 12 h
			Indirect	Western blot - wt vs TF mutant	Positive	BJ1991	Cells grown in YPD (0.05% glucose) medium with 0.2% oleic acid
		PubMed Smith JJ et al., Mol Syst Biol, 2007;():	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4742 or BY4739	5-h induction in oleate
		PubMed Karpichev IV et al., J Biol Chem, 2008 Apr 18;283(16):10264-75	Direct	ChIP	N/A	S288c	Exponential phase cells cultivated in YP growth medium containing 2% glycerol as carbon source
		PubMed Thakur JK et al., J Biol Chem, 2009 Feb 13;284(7):4422-8	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	oleic acid-containing medium
		PubMed Ratnakumar S et al., J Biol Chem, 2010 Apr 2;285(14):10703-14	Direct	ChIP	N/A	S288c	Glucose derepression; Exponential-phase Dpip2 cells cultivated for 1 h in synthetic growth medium containing 0.1% oleate + 3% glycerol
		PubMed Wan Y et al., Biochem J, 2013 Jan 15;449(2):507-17	Direct	ChIP	N/A	S288c	induction in oleate (1-6 h)
		PubMed Parua PK et al., J Biol Chem, 2014 Dec 19;289(51):35542-60	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YP medium + 0.1% OLEATE
		PubMed Bergenholm D et al., mSystems, 2018 Jul-Aug;3(4):	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Steady state aerobic chemostat cultivation at 30 .C in nitrogen-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Steady state aerobic chemostat cultivation at 30 .C in glucose-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Steady state chemostat cultivation at 30 .C in oxygen- and glucose-limited minimal medium containing vitamins and trace metals and maintained at pH 5.0
		PubMed Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h