S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Pho4p	YBR296C	PubMed Ogawa N et al., Mol Biol Cell, 2000 Dec;11(12):4309-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Exponential phase cells cultivated in YPD
		PubMed Serrano R et al., Mol Microbiol, 2002 Dec;46(5):1319-33	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Alkaline pH stress; Cells cultivated in SC medium at pH 8
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Nishizawa M et al., PLoS Biol, 2008 Dec 23;6(12):2817-30	Direct	Chip-on-chip	N/A	S288c	YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)
		PubMed Dos Santos SC et al., Mol Genet Genomics, 2011;():	Indirect	lacZ - wt vs TF mutant	Positive	S288c	quinine stress
		PubMed Aow JS et al., Nucleic Acids Res, 2013 May;41(9):4877-87	Direct	ChIP	N/A	S288c	low phosphate
		PubMed Serra-Cardona A et al., Mol Cell Biol, 2014 Dec;34(24):4420-35	Indirect	Western blot - wt vs TF mutant	Positive	S288c	Cells were subjected to pH 8.0 for 90, 120, 180 min followed by immunoblotting using anti-HA antibodies to reveal HA-tagged Pho89
		PubMed He et al., Elife, 2017;6(0):e25157He et al., Elife, 2017;6(0):e25157	Indirect	RNA-seq - wt vs TF mutant	Positive	EY0057 pho80DELTA	EY0057 pho80DELTA; Minimal medium; mid-log phase
		PubMed Gurvich Y et al., PLoS Biol, 2017 Dec 13;15(12):e2002039	Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741	BY4741; SC medium; Phosphate(i) starvation condition; 30.C; mid-log phase
			Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO84-constitutive	BY4741 PHO84c; SC medium; yeast cell recovery into 20mM P(i) from low P(i); 30.C; 2h growth