S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Rtg1p	YCR005C	PubMed Liao X et al., Cell, 1993 Jan 15;72(1):61-71	Direct	lacZ - wt vs mutant promoter sequence	N/A	S288c	N/A
		PubMed Jia Y et al., Mol Cell Biol, 1997 Mar;17(3):1110-7	Direct	EMSA	N/A	S288c	in vitro
		PubMed Liu Z et al., Mol Cell Biol, 1999 Oct;19(10):6720-8	Indirect	Northern blot - wt vs TF mutant	Positive	PSY142	rho0 cells cultivated in YP medium with 2% raffinose
		PubMed Epstein CB et al., Mol Biol Cell, 2001 Feb;12(2):297-308	Indirect	Microarray analysis - wt vs TF mutant	Positive	PSY142 (MATALFA leu2 lys2 ura3rho+)	Mitochondrial dysfunction; rho0 petites grown in YP raffinose (2%) medium to exponential phase
		PubMed Guaragnella N et al., J Biol Chem, 2003 Nov 14;278(46):45882-7	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	YPRaffinose medium
		PubMed Chen L et al., Yeast, 2010 Jun;27(6):345-59	Indirect	lacZ - wt vs TF mutant	Positive	BY4742	CIT2-lacZ reporter gene was transformed into a wt vs isogenic bHLH knock-out RO+ strains (DELTArtg1 with mitochondrial DNA). CIT2-lacZ expression was assayed from cultures grown in the absence (I-C-) or presence of inositol and choline (I+C+) containing either low-phosphate (Pi low) or high-phosphate (Pi high).
		PubMed Chen L et al., Yeast, 2010 Jun;27(6):345-59	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4742	CIT2-lacZ reporter gene was transformed into a wt vs isogenic bHLH knock-out RO+ strains (DELTArtg1 with mitochondrial DNA). CIT2-lacZ expression was assayed from cultures grown in the absence (I-C-) or presence of inositol and choline (I+C+) containing either low-phosphate (Pi low) or high-phosphate (Pi high).
		PubMed Lin AP et al., J Biol Chem, 2011 Sep 30;286(39):33737-46	Indirect	Western blot - wt vs TF mutant	Positive	S288c	YPGal medium; mid-log phase
		PubMed Ruiz-Roig C et al., Mol Biol Cell, 2012 Nov;23(21):4286-96	Direct	ChIP	N/A	S288c	NaCl (osmotic stress)
		PubMed Kingsbury JM et al., Hum Mol Genet, 2016 Aug 15;25(16):3524-3538	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	cells with IDP1R148H (analogous to human Idh1R132) with strong GAL1 promoter, grown in Synthetic Complete Galactose 24h of incubation, show the same effect when compared to WT (S288c) cells.
		PubMed Dey T et al., J Biol Chem, 2018 Oct 26;293(43):16647-16660	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	DELTArtg1 vs DELTArtg1+overIndirect of RTG1 gene: strains cultured in YNBD Glutamate- or YNBD Glutamate+ media
		PubMed Romero AM et al., Biochim Biophys Acta Gene Regul Mech, 2019 Sep;1862(9):194414	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	30.C in SC (+Fe) and SC with 100 microM BPS (-Fe) for 9 hours
			Direct	RNA Pol II ChIP WT vs TF mutant	Positive	BY4741	At time zero, 100 microM BPS was added to cells exponentially growing in SC at 30.C
		PubMed Hijazi I et al., J Cell Sci, 2020 Mar 30;133(6):	Indirect	lacZ - wt vs TF mutant	Positive	BY4742/BY4741	SC medium
			Indirect	lacZ - wt vs TF mutant	Positive	BY4742/BY4741	SC medium + 0.5 microg/mL tunicamycin for 5 h
			Indirect	lacZ - wt vs TF mutant	Positive	BY4742/BY4741	SC medium + 1 mM DTT for 5 h
			Indirect	lacZ - wt vs TF mutant	Positive	BY4742/BY4741	SC medium + 200 nM rapamycin for 5 h
		PubMed , , ;():, , ;():	Indirect	Western blot - wt vs TF mutant	Positive	W303-1B	YPR medium: 1% Yeast extract (DIFCO), 2% Bacto Peptone (DIFCO), 2% raffinose