S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Ste12p	YBR040W	PubMed Madhani HD et al., Proc Natl Acad Sci U S A, 1999 Oct 26;96(22):12530-5	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	haploid-invasive growth conditions; Haploid cells grown in YPD rich medium
		PubMed Urnov FD, Biochem Cell Biol, 2002;80(3):321-33	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Zeitlinger J et al., Cell, 2003 May 2;113(3):395-404	Direct	Chip-on-chip	N/A	S288c	YPD medium + alpha-factor
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Heise B et al., Eukaryot Cell, 2010 Apr;9(4):514-31	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	SC medium; mid-log phase
		PubMed Zheng W et al., Nature, 2010 Apr 22;464(7292):1187-91	Direct	ChIP-seq	N/A	S288c	YPD medium + alpha-factor
		PubMed Arroyo J et al., Mol Microbiol, 2011;():	Indirect	RT-PCR - wt vs TF mutant	Negative	S288c	Yeast strains were grown in YPDt o an optical density (OD600) of 0.1. Cultures were split up and treated with OGT2468 at final concentrations of 0.1 µM or 1 µM, and DMSO (final concentration 1%) for 3h
		PubMed Aymoz D et al., Mol Syst Biol, 2018 Apr 25;14(4):e8024	Direct	ChIP	N/A	W303	Yeast cultures were grown to early log phase (A660 0.4-0.6) and then subjected to 1 microM alfa-factor