PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Zheng W et al., Nature, 2010 Apr 22;464(7292):1187-91Zheng W et al., Nature, 2010 Apr 22;464(7292):1187-91
Direct
ChIP-seq
N/A
S288c
YPD medium + alpha-factor
PubMed
Chavel CA et al., PLoS Genet, 2010 Mar 19;6(3):e1000883Chavel CA et al., PLoS Genet, 2010 Mar 19;6(3):e1000883
Indirect
Microarray analysis - wt vs TF mutant
Negative
SIGMA1278b
wt and DELTAste12 cells cells containing an activated allele of MSB2 were grown in YEP-GAL for 6h, at which point cells were examined by microscopy for the characteristic filamentation response