PubMed
Birkaya B et al., Eukaryot Cell, 2009 Aug;8(8):1118-33Birkaya B et al., Eukaryot Cell, 2009 Aug;8(8):1118-33
Indirect
Northern blot - wt vs TF mutant
Positive
S288c
cells grown in YP Galactose to mid-log phase
PubMed
Zheng W et al., Nature, 2010 Apr 22;464(7292):1187-91Zheng W et al., Nature, 2010 Apr 22;464(7292):1187-91
Direct
ChIP-seq
N/A
S288c
YPD medium + alpha-factor
PubMed
Chavel CA et al., PLoS Genet, 2010 Mar 19;6(3):e1000883Chavel CA et al., PLoS Genet, 2010 Mar 19;6(3):e1000883
Indirect
Microarray analysis - wt vs TF mutant
Positive
SIGMA1278b
wt and DELTAste12 cells cells containing an activated allele of MSB2 were grown in YEP-GAL for 6h, at which point cells were examined by microscopy for the characteristic filamentation response
PubMed
Chow J et al., J Fungi (Basel), 2018 Aug 9;4(3):93Chow J et al., J Fungi (Basel), 2018 Aug 9;4(3):93
Indirect
RT-PCR - wt vs TF mutant
Positive
SIGMA1978b
YEP-Gal (2% agarose) for 24h
PubMed
Chow J et al., Genetics, 2019 Jul;212(3):667-690Chow J et al., Genetics, 2019 Jul;212(3):667-690