S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Ste12p	YNL279W	PubMed Urnov FD, Biochem Cell Biol, 2002;80(3):321-33	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Zeitlinger J et al., Cell, 2003 May 2;113(3):395-404	Direct	Chip-on-chip	N/A	S288c	YPD medium + alpha-factor
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed LefranÃ§ois P et al., BMC Genomics, 2009;():	Direct	ChIP-seq	N/A	S288c	Pseudohyphal growth conditions (nitrogen depleted SLAD medium)
		PubMed Heise B et al., Eukaryot Cell, 2010 Apr;9(4):514-31	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	SC medium; mid-log phase
		PubMed Zheng W et al., Nature, 2010 Apr 22;464(7292):1187-91	Direct	ChIP-seq	N/A	S288c	YPD medium + alpha-factor
		PubMed HÃ¶fken T, Biochem Biophys Res Commun, 2017 Dec 2;493(4):1485-1490	Indirect	lacZ - wt vs TF mutant	Positive	THY612	wt and DELTAste12 cells carrying a PRM1-lacZ plasmid were grown in synthetic complete (SC) medium
			Indirect	lacZ - wt vs TF mutant	Positive	THY612	wt and STE12-overexpressing cells carrying a PRM1-lacZ plasmid were grown in medium with 2% galactose and 3% raffinose instead of glucose.
			Indirect	lacZ - wt vs TF mutant	Positive	THY612	Cells harboring the PRM1-lacZ plasmid vs the PRM1 promoter lacking the three Ste-12-binding sites were incubated with 10 mg/ml alpha-factor for 150 min to test response to pheromone
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C