S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Stp2p	YBR068C	PubMed de Boer M et al., Nucleic Acids Res, 2000 Feb 15;28(4):974-81	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	2mM leucine
		PubMed Nielsen PS et al., Mol Gen Genet, 2001 Jan;264(5):613-22	Direct	EMSA	N/A	S288c	N/A
		PubMed Wielemans K et al., J Biol Chem, 2010 Jan 8;285(2):855-65	Indirect	lacZ - wt vs TF mutant	Positive	S288c	5mM (high) Phenylalanine
		PubMed Tanaka N et al., Biochem Biophys Res Commun, 2015;():	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	YPD + tryptophan (1mg/mL)
		PubMed Muñoz SA et al., Microbiology (Reading), 2020 Jan;166(1):85-92	Indirect	lacZ - wt vs TF mutant	Positive	KW021	minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM ammonium sulfate as a nitrogen source
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM ammonium sulfate as a nitrogen source + 1.3mM leucine for 1h
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM proline as a nitrogen source
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM proline as a nitrogen source + 1.3mM leucine for 1h
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour.
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.
			Indirect	lacZ - wt vs TF mutant	Positive	KW021	Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour.
			Indirect	LacZ - WT vs. TF double mutant	Positive	KW022	Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.
			Indirect	LacZ - WT vs. TF double mutant	Positive	KW022	Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour.
			Indirect	LacZ - WT vs. TF double mutant	Positive	KW022	Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.
			Indirect	LacZ - WT vs. TF double mutant	Positive	KW022	Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour.
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C