S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Upc2p	YJL105W	PubMed Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
		PubMed Serratore ND et al., Genetics, 2018 Mar;208(3):1037-1055	Indirect	RT-PCR - wt vs TF mutant	Positive	W303 and/or S288C strains (I do not have access to the supplementary data of the article)	wt, DELTAupc2 and DELTAupc2DELTAecm22 cells were grown in SC medium at 30.C, in hypoxia conditions
			Indirect	RT-PCR - wt vs TF mutant	Positive	W303 and/or S288C strains (I do not have access to the supplementary data of the article)	wt, DELTAupc2 and DELTAupc2DELTAecm22 cells were grown in SC medium at 30.C. Cultures were treated at an OD600: 0.2 with 56 microg/mL of ketoconazole (Sigma-Aldrich) dissolved in DMSO.
			Indirect	RT-PCR - wt vs TF mutant	Positive	W303 and/or S288C strains (I do not have access to the supplementary data of the article)	DELTAerg3 and DELTAupc2DELTAerg3 cells were grown in SC medium at 30.C, in hypoxia conditions.
			Indirect	Western blot - wt vs TF mutant	Positive	W303 and/or S288C strains (I do not have access to the supplementary data of the article)	wt, DELTAupc2 and DELTAupc2DELTAecm22 cells were grown in SC medium at 30.C, in hypoxia conditions. For immunoprecipitation, 60 microL of Protein-G magnetic Dynabeads were conjugated with 20 microL M2 FLAG antibody and immunoprecipitated with 2 mL of whole cell lysate for 2 hours rotating at 4.C.
			Indirect	Western blot - wt vs TF mutant	Positive	W303 and/or S288C strains (I do not have access to the supplementary data of the article)	wt, DELTAupc2 and DELTAupc2DELTAecm22 cells were grown in SC medium at 30.C. Cultures were treated at an OD600: 0.2 with 56 microg/mL of ketoconazole (Sigma-Aldrich) dissolved in DMSO. For immunoprecipitation, 60 microL of Protein-G magnetic Dynabeads were conjugated with 20 microL M2 FLAG antibody and immunoprecipitated with 2 mL of whole cell lysate for 2 hours rotating at 4.C.
			Indirect	Western blot - wt vs TF mutant	Positive	W303 and/or S288C strains (I do not have access to the supplementary data of the article)	DELTAerg3 and DELTAupc2DELTAerg3 cells were grown in SC medium at 30.C, in hypoxia conditions. For immunoprecipitation, 60 microL of Protein-G magnetic Dynabeads were conjugated with 20 microL M2 FLAG antibody and immunoprecipitated with 2 mL of whole cell lysate for 2 hours rotating at 4.C.