S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Upc2p	YMR202W	PubMed Vik A et al., Mol Cell Biol, 2001 Oct;21(19):6395-405	Direct	EMSA	N/A	W303-1a	In vitro; interaction between DNA probes containing sequences from the ERG2 promoter and a truncated Ecm22p(1-497) protein
			Indirect	Northern blot - wt vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with 40 microg/mL lovastatin
			Indirect	Northern blot - wt vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with or without 40 microg/mL lovastatin
			Indirect	lacZ - wt vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with 40 microg/mL lovastatin; lacZ regulated only by the sterol regulatory element (SRE) of ERG2
			Indirect	lacZ - wt vs TF mutant	Positive	W303-1a	Cells grown in minimal medium (16 h) with or without 40 microg/mL lovastatin
		PubMed Davies BS et al., Mol Cell Biol, 2005 Aug;25(16):7375-85	Indirect	lacZ - wt vs TF mutant	Negative	JRY7179	Complete synthetic medium (CSM; 0.67%Difco yeast nitrogen base without amino acids, complete supplemental mixture minus appropriate amino acids) containing 2% glucose; mid-log phase.
			Indirect	BETA-galactosidase (WT vs TF mutant)	Positive	JRY7179	Complete synthetic medium (CSM; 0.67%Difco yeast nitrogen base without amino acids, complete supplemental mixture minus appropriate amino acids) containing 2% glucose; mid-log phase.
		PubMed Davies BS et al., Genetics, 2006 Sep;174(1):191-201	Indirect	RT-PCR - wt vs TF mutant	Positive	W303	Cells were grown aerobically in complete synthetic medium containing 2% glucose with ketoconazole
			Indirect	RT-PCR - wt vs TF mutant	Negative	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions)
			Indirect	RT-PCR - wt vs TF mutant	Positive	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions) with hemin
			Indirect	RT-qPCR (WT vs TF mutant)	Negative	W303	Cells were grown in complete synthetic medium containing 2% glucose in tightly capped 50 ml flasks without shaking at 30. (hypoxic conditions) with hemin
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed MontaÃ±Ã©s FM et al., Mol Microbiol, 2011 Feb;79(4):1008-23	Indirect	Northern blot - wt vs TF overexpression	Positive	S288c	YPD medium; mid-log phase
		PubMed Yang H et al., Nat Commun, 2015;():Yang H et al., Nat Commun, 2015;():	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Cells expressing pRS413-ERG2-lacZ and YCplac33-UPC2 plasmids exposed to fluconazole 4ug/mL