S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Yap1p	YGR088W	PubMed Lee J et al., J Biol Chem, 1999 Jun 4;274(23):16040-6	Indirect	Proteomics - wt vs TF mutant	Positive	S288c	15 minutes exposure to 0.2 mM H2O2 (SC growth medium)
		PubMed Hasan R et al., Mol Microbiol, 2002 Jul;45(1):233-41	Indirect	Proteomics - wt vs TF mutant	Positive	YPH98	Oxidative stess; cells grown in YNB medium to exponential phase exposed to 0.5 mM H2O2 for 30 min
		PubMed Wysocki R et al., Mol Biol Cell, 2004 May;15(5):2049-60	Indirect	Northern blot - wt vs TF mutant	N/A	S288c	N/A
		PubMed Bourges I et al., J Biol Chem, 2005 Aug 19;280(33):29743-9	Indirect	Microarray analysis - wt vs TF overexpression	Negative	S288c	Myxothiazol - Cells were cultivated in YPGal for 7 h and 50 min. Acetoin or acetaldehyde were added at concentrations of 10 mM, and the cells were incubated for 10 min. 8 M myxothiazol was then added, and the cells were incubated for another 10 min
		PubMed Thorsen M et al., Physiol Genomics, 2007 Jun 19;30(1):35-43	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	0.2 mM As(III), 1 h
		PubMed Salin H et al., BMC Genomics, 2008 Jul 15;0(0):33	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4742	YPD+1mM selenite 40 min
			Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4742	YPD+1mM selenite 60 min
			Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4742	YPD+1mM selenite 80 min
		PubMed Ouyang X et al., Free Radic Biol Med, 2011 Jan 1;50(1):1-13	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	H2O2 treatment
		PubMed Zhang C et al., Yeast, 2016 Jan;33(1):5-19	Indirect	RNA-seq - wt vs TF mutant	Positive	S288c	SD medium + t-BHP
		PubMed Shedlovskiy D et al., J Biol Chem, 2017 Nov 10;292(45):18469-18485	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	YPDA medium (1% yeast extract, 2% peptone, 2% dextrose, 10 mg/L adenine) treated with 0.2 mM H2O2 (oxidative stress) for 240 and 480 min
		PubMed Nakazawa N et al., J Gen Appl Microbiol, 2018 Sep 27;64(4):149-157	Indirect	Northern blot - wt vs TF mutant	Positive	Shirakami kodama yeast (S. cerevisiae) IB1306 MATalpha ho	Cells grown in supplemented synthetic medium (SD, 0.67% yeast nitrogen base without amino acids, 2% glucose, and a supplement with amino acids).
			Indirect	Northern blot - wt vs TF mutant	Positive	Shirakami kodama yeast (S. cerevisiae) IB1306 MATalpha ho	Cells grown in supplemented synthetic medium (SD, 0.67% yeast nitrogen base without amino acids, 2% glucose, and a supplement with amino acids) treated with 0.3 mM H2O2 for 30 min at 30 .C (oxidative stress)