S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Yap1p	YLR109W	PubMed Lee J et al., J Biol Chem, 1999 Feb 19;274(8):4537-44	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Oxidative stress - Yeast cells from overnight culture were diluted to an OD600 of 0.01 in SC medium and incubated with shaking at 30 °C until they reached an OD of 0.3. The cells were then aliquoted and incubated in the absence or in the presence of t-BOOH (0.2 mM) for 20 min.
		PubMed Lee J et al., J Biol Chem, 1999 Jun 4;274(23):16040-6	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	20 minutes exposure to 0.2 mM H2O2 (SC growth medium)
		PubMed Rep M et al., Mol Microbiol, 2001 Jun;40(5):1067-83	Indirect	Northern blot - wt vs TF mutant	Positive	CEN.PK2-1C	Oxidative stress; 0.1 mM H2O2 added to cells grown in YPD medium to mid-exponential phase
		PubMed Hasan R et al., Mol Microbiol, 2002 Jul;45(1):233-41	Indirect	Proteomics - wt vs TF mutant	Positive	YPH98	Oxidative stess; cells grown in YNB medium to exponential phase exposed to 0.5 mM H2O2 for 30 min
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium + 200mM hydroxyurea
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Thorsen M et al., Physiol Genomics, 2007 Jun 19;30(1):35-43	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	0.2 mM As(III), 1 h
		PubMed Salin H et al., BMC Genomics, 2008 Jul 15;0(0):33	Direct	Chip-on-chip	N/A	BY4742	YPD + Sodium selenite (1 mM), hydrogen peroxide (0.3 mM) or benomyl (20 microg/ml)
			Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4742	YPD
			Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4742	YPD+1mM selenite 40 min
			Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4742	YPD+1mM selenite 60 min
			Indirect	Microarray analysis - wt vs TF mutant	Negative	BY4742	YPD+1mM selenite 80 min
		PubMed Kelley R et al., PLoS Genet, 2009 May;5(5):e1000488	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD 30.C 0.1 mM H2O2
		PubMed Kelley R et al., PLoS Genet, 2009 May;5(5):e1000488	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YPD 30.C from 0.1 mM H2O2 to 0.4 mM H2O2
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed EscotÃ© X et al., Yeast, 2012 Jul;29(7):251-63	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	resveratrol stress
		PubMed Weiner A et al., PLoS Biol, 2012;10(7):e1001369	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Pimentel C et al., Biochim Biophys Acta, 2014 Jun;1840(6):1977-86	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	2 mM CoSO4
		PubMed Zhang C et al., Yeast, 2016 Jan;33(1):5-19	Indirect	RNA-seq - wt vs TF mutant	Positive	S288c	SD medium + t-BHP + MMS
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C