PubMed
Pramila T et al., Genes Dev, 2002 Dec 1;16(23):3034-45Pramila T et al., Genes Dev, 2002 Dec 1;16(23):3034-45
Indirect
Microarray analysis - wt vs TF mutant
Negative
W303
Cells grown in YPD medium synchronised with 5 microg/mL ALFA-factor and monitored at 10 min intervals for 2 h
Indirect
Microarray analysis - wt vs TF overexpression
Negative
W303
Cells grow in YPD medium before overexpressing Yox1p for 4 h
PubMed
Bastajian N et al., PLoS Genet, 2013 May;9(5):e1003507Bastajian N et al., PLoS Genet, 2013 May;9(5):e1003507
Direct
ChIP
N/A
S288c
Cells with YOX1-TAP carrying or not pGAL-BCK2-FLAG plasmid, were grown in plasmid selective medium containing galactose to mid-log phase. Cultures were harvested and anti-TAP ChIPs were performed using IgG sepharose resin and analyzed for CLN3 promoter DNA using qPCR.